Antibacterial Activity Test of Ethanol Extract Combination of Moringa Leaves (Moringa Oleifera Lam) and Binahong Leaves (Anredera Cordifolia) Against Bacteria Escherichia Coli Atcc 25922

Abstrak

Moringa leaf extract and binahong leaf contain alkaloids, flavonoids, phenol, tannins, saponins, and triterpenoid compounds as antibacterial. This study aimed to determine the antibacterial activity of the combination of ethanol extract of moringa leaves and binahong leaves on the growth of Escherichia coli ATCC 25922 bacteria. The extract was made using the maceration method with 70% ethanol solvent, and phytochemical testing was carried out. Determination of the Minimum Killing Concentration of moringa leaf extract and binahong leaves using the dilution method. The combination of moringa leaf extract and binahong leaf was made with a ratio of (1:1), (1:2), (2:1). Determination of antibacterial activity was performed using the disc diffusion method and the results of the inhibition zone
diameter were analyzed using SPSS. The combination effect
pattern was carried out using the paper tape method. The results
showed that moringa leaf extract had a Minimum Killing
Concentration value of 6.25% and binahong leaves 12.5%. The 1:1
combination has an inhibitory power of 14.73 ± 0.50. The
combination of 1:2 is 14.96±0.45. The combination of 2:1 is
16.2±0.55. The combination of 2:1 is the most effective in
inhibiting the growth of E. coli bacteria and provides a synergistic
combination pattern.