Antihyperglycemic Activity Test of Javanese Pepper Leaf Ethanol Extract (Piper Retrofractum Vahl) And Pancreatic Histopathology in Male White Mice (Mus Musculus) By Aloxane Induction

Abstrak

Diabetes mellitus is a metabolic disease that causes hyperglycemia. Javanese chili (Piper retrofractum Vahl) contains flavonoids, alkaloids, tannins and saponins that are efficacious as a blood sugar level lowerer. The purpose of this study is to determine the activity and effective dose of antihyperglycemia of javanese chili leaf extract, as well as to determine the ability to improve pancreatic histopathology in male mice that have been induced by aloxan. The maceration extraction of Javanese chili leaves is carried out with a 70% ethanol solvent. As many as 30 Mus musculus mice were induced with 1% aloxan, then given Na CMC negative control treatment, glibenchamide positive control, extract treatment dose of 1.4, 2.8, 5.6 mg/kgBB mice. Blood sugar levels were measured before and after treatment with an easy test glucometer, then mice were sacrificed and their pancreas were taken for histopathological testing. The data was tested using the One Way Anova and Paired T-test. The results showed that all javanese chili leaf extracts could reduce blood sugar levels and significantly reduce the percentage of necrosis (Sig. <0.05).