Testing The Antioxidant Activity Nasturtium Flower Extract (Tropaeolum Majus L.) Nanosuspension Using of Dpph Methods

Abstrak

The increasing incidence of diseases caused by free radicals encourages research on antioxidant compounds, one of which is from nasturtium flowers (Tropaeolum majus L.) which contains phenolic compounds. This study aims to increase the antioxidant activity of nasturtium flower extract through nanoparticle technology in the form of nanosuspension, which is made by sonoprecipitation method using a variety of stabilizers. After extraction, compound identification, and determination of total phenolics and flavonoids, the nanosuspension characteristics were tested, including particle size and antioxidant activity using the DPPH method. The results showed that nanosuspensions of nasturtium flower extract could be produced with particle sizes ranging from 989.5-1214.9 nm, with the best stabilizer being Pluronic F-68. Antioxidant activity test shows that formula 2 has an IC50 value of 1866.8897 ppm, lower than the pure extract which has an IC50 value of 2842.3616 ppm. In conclusion, nasturtium flower extract nanosuspension is effective in increasing antioxidant activity.