The Antibacterial Activity Test of the Combination of Ethanol Extracts from Moringa Leaves (Moringa oleifera Lam) and Belimbing Wuluh Leaves (Averrhoa bilimbi Linn) Against Staphylococcus aureus ATCC 25923

Abstrak

Moringa leaves (Moringa oleifera L.) and star fruit leaves (Averrhoa bilimbi Linn) contain chemical compounds such as flavonoids, saponins, tannins, triterpenoids, and essential oils, all of which have demonstrated antibacterial activity. Previous research has indicated that moringa and star fruit leaves exhibit antibacterial activity against Staphylococcus aureus ATCC 25923. This study aimed to determine which extract of moringa leaves, star fruit leaves, or their combination has the most effective antibacterial activity in inhibiting the growth of Staphylococcus aureus ATCC 25923. The extraction method used in this study involved a 1:10 ratio with 70% ethanol as the solvent. Antibacterial testing was conducted using the dilution method with concentrations of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.758%, and 0.39%, the diffusion method (disc method), and paper tape to assess the combination pattern. The concentration ratios used for the combination were 1:1, 1:2, and 2:1, with ciprofloxacin as a positive control. The results showed that the combination of ethanol extracts of moringa leaf and star fruit exhibited antibacterial activity against Staphylococcus aureus ATCC 25923. Moringa leaf extract and star fruit extract demonstrated antibacterial activity at a concentration of 6.25%, with inhibitory zone diameters of 15.52 mm and 14.21 mm, respectively. The most effective combination ratio of moringa leaf and star fruit ethanol extract was 2:1, resulting in an inhibitory zone diameter of 19.57 mm. The combination effect was found to be synergistic.