Analgesic Activity Test of Ethanol Extract of Lempuyang Gajah Rhizome (Zingiber zerumbet (L.) Roscoe Ex Sm.) on Male White Mice (Mus musculus) Using Tail Flick and Writhing Test Methods

Abstract

Pain is an abnormal sensory and emotional event associated with tissue damage. Elephant rhizome of (Zingiber zerumbet (L.) Roscoe ex Sm.) can be utilized as an alternative pain treatment. The purpose of this study was to determine whether ethanol extract of Zingiber zerumbet rhizome has analgesic activity and what is the effective dose as an analgesic. Elephant Zingiber zerumbet rhizome was extracted by maceration with 96% ethanol solvent. Test animals were divided into 5 treatment groups: negative control group (CMC Na), positive control group (tramadol and mefenamic acid), extract group (doses of 25 mg/kgBB, 50 mg/kgBB, and 100 mg/kgBB). Tests using the Tail Flick and Writhing Test methods. Data analysis using Shapiro- Wilk test, Levene test, One Way Anova followed by Post Hoc. The results showed that ethanol extract of Zingiber zerumbet can provide analgesic activity with an effective dose of 100 mg/kgBB mice.