In Vitro Antioxidant Activity Testing of Mangrove Leaves Ethanol Extract (Rhizophora mucronata) and Antihyperglycemia Activity Testing in Mice (Mus musculus L.)

Abstract

Mangrove leaves (Rhizophora mucronata) have chemical content, flavonoids, tannins, triterpenoids, saponins and alkaloids. The purpose this study was to determine the ethanol extract mangrove leaves has antioxidant activity and reduce blood glucose levels in male white mice induced by alloxan. Antioxidant activity testing using the DPPH method with quercetin comparator standard and antihyperglycemia activity testing which divided into 6 groups, Group I negative control (0.5% CMC suspension), group II positive control (glibenclamide 0.65 mg/kg BB), group III mangrove leaf extract dose (31.25 mg/kg BB), group IV mangrove leaf extract dose (62.5 mg/kg BB), group V mangrove leaf extract dose (125 mg/kg BB), and group VI normal control. The results showed that mangrove leaf extract has antioxidant activity with an IC50 value of 29.05 ppm having a very strong katergori. The effective dose of mangrove leaf extract that can reduce blood glucose levels is in group IV mangrove leaf extract at a dose of 31.25 mg/kg BB.