Network Pharmacology of Pulai Leaves (Alstonia scholaris L.) as Antiasthma

Abstract

Asthma is a heterogeneous disease of the lower airway. This study aims to identify protein involved in the pathophysiology of asthma, as well as evaluate the potential of pulai leaves as anti- asthma through pharmacological network analysis of target proteins. This research uses the NP method to collect data on pulai leaf chemical compounds from KNApSAcK. Asthma target proteins were identified through the KEGG pathway and validated with UniProt. Protein interactions used String, active substance screening was done through PubChem, Swiss Target Prediction, SEA, and SuperPred, and interaction visualization was done with Cytoscape. Visualization of NP profiles showed asthma target proteins involved with pulai leaf compounds, namely NFKB1, GSK3B, SYK, BLNK, BTK, GRB2, LAT, LCK, MAP2K1, RAF1, PIK3CA, and FYN. Compounds such as aquammicine, betulinic acid, oleanolic acid, ursolic acid, beta-sitosterol, isorhamnetin, chlorogenic acid, quercetin, citral, tetrahydroalstonine, alpha- amyrin, and kaempferol in pulai leave form a NP profile with asthma target protein.