Antibacterial Activity Test from Metabolites of Endophyte Bacteria of Gemitir Flowers (Tagates Erecta L.) Against Bacteria Staphyloccus Aureus

Abstract

The search for sources of bioactive compounds continues to be carried out along with the emergence of new diseases, the existence of resistance to antibiotics leads to the treatment of infections caused. One source of bioactive compounds used to fight antibiotic resistance is endophytic microbes. Endophytic bacteria can produce chemical compounds that have effects on health, especially endophytic bacteria isolated from the medicinal plant of the gemitir flower (Tagetes erecta L.). Plant determination is followed by sterilization of tools and materials then gemitir flowers are isolated in planted in Nutrient Agar (NA). are Vogel Johnson Agar (VJA), Mueller Hilton Agar (MHA), Nutrient Agar (NA) and Brain Heart Infusion (BHI), Media SIM, Media KIA, Media LIA, Media Citrate. The results showed that as many as 5 metabolites of endophytic bacteria were successfully isolated from the gemitir flower (Tagetes erecta L). Gemitir endophytes have colonies with sizes and shapes, namely circular and irregular, and the shape of the edges is undulate, and entire. The surface of the colony shows a shaped elevation that is flat and raised. Gram painting of 5 different Metabolites shows 1 Gram positive endophytic bacterial metabolite, namely in EG1 Metabolites While Gram negative 4 Metabolites, namely EG2, EG3, EG4, and EG5. The genus of bacteria found are Bacillus and Pseudomonas. The inhibition zones were 12.1 mm, 11 mm, and 9.53 mm respectively from 24-hour, 48-hour, and 72-hour fermentation. EG1 metabolites in the 24 hour group showed the highest antibacterial activity.