Antibacterial Activity Test of Betadine Leaf Extract and Fraction (Jatropha multifida L) against Staphylococcus aureus and Pseudomonas aeruginosa bacteria

Abstract

This study aims to determine the most active antibacterial activity of Betadine leaf extract and fraction against Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Betadine leaf extract using maceration method with 96% ethanol solvent and fractionation using n -hexane, ethyl acetate and water solvents. Antibacterial activity test using disc diffusion method and dilution method. The results showed that betadine leaf extract and fraction have antibacterial activity. The diffusion results showed that the ethyl acetate fraction with a concentration of 50% had the most active antibacterial activity with an average diameter of 14.40 mm (50%); 13.17 mm (25%); 10.47 mm (12.5%) in Staphylococcus aureus and 10.8 mm (50%); 10.5 mm (25%); 8.37 mm (12.5%) on Pseudomonas aeruginosa . From the dilution method, MIC could not be determined because the solution was too cloudy, but MBC was determined at a concentration of 3.12% against Staphylococcus aureus bacteria and a concentration of 25% against Pseudomonas aeruginosa .